Yesterday I conducted an experiment with Biuret Reagent, am indicator of peptide bonds that transmutes into a purple color when in contact with a protein. I did this with a goal to identify if almonds genuinely did have a large amount of protein and was a viable source of it during the day.
In order to conduct the test, I used water the Biuret reagent, protein powder as a positive control, water as a negative control, and crushed almonds.
The first step was create an aqueous solution with the substance being tested in it or water that has been decanted out of it. This can be done by crushing the substance, putting it in water, and after letting it sit, and decanting the water out. This means slowly pouting the water out without letting the substance get in.
Next, you put an equal volume of Biuret reagent in the water. Mix the reagent in until a color change occurs. The concentration of purple in the new solution reflects the concentration of peptide bonds in your substance, and thus it reflects the amount of protein in it. the deeper the purple, the greater the protein content.
Now back to the test I conducted. The protein powder turned dark purple and the water turned blue as expected. Then, I tested the almonds and I found it turned clear purple. This ascertained that almonds do in fact have a high protein content in them. Along with this, it showed that the protein powder had a even greater amount of protein that almonds.
In the next few days, I plan to conduct an experiment to test for the concentration of sugar in a substance, however this time I will go further by trying to calculate the actual amount of sugar in the substance. I will be testing chocolate syrup because it will be easy to turn into an aqueous solution. I hope you learned a little from this post about the way to measure protein content in food and encourage you to try it yourself
In order to conduct the test, I used water the Biuret reagent, protein powder as a positive control, water as a negative control, and crushed almonds.
The first step was create an aqueous solution with the substance being tested in it or water that has been decanted out of it. This can be done by crushing the substance, putting it in water, and after letting it sit, and decanting the water out. This means slowly pouting the water out without letting the substance get in.
Next, you put an equal volume of Biuret reagent in the water. Mix the reagent in until a color change occurs. The concentration of purple in the new solution reflects the concentration of peptide bonds in your substance, and thus it reflects the amount of protein in it. the deeper the purple, the greater the protein content.
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The liquids in these beakers are water, dosa batter (Indian bread), almonds, and protein powder
(left to right)
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Now back to the test I conducted. The protein powder turned dark purple and the water turned blue as expected. Then, I tested the almonds and I found it turned clear purple. This ascertained that almonds do in fact have a high protein content in them. Along with this, it showed that the protein powder had a even greater amount of protein that almonds.
In the next few days, I plan to conduct an experiment to test for the concentration of sugar in a substance, however this time I will go further by trying to calculate the actual amount of sugar in the substance. I will be testing chocolate syrup because it will be easy to turn into an aqueous solution. I hope you learned a little from this post about the way to measure protein content in food and encourage you to try it yourself
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